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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
02/01/2017 |
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Actualizado : |
02/01/2017 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
CASTILLO, A.; MONTAÑEZ MASSA, A.; DOCAMPO, R.; RODRIGUEZ, P.; CABRERA, D.; ZOPPOLO, R. |
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Afiliación : |
ALICIA MARIA CASTILLO SALLE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ADRIANA MONTAÑEZ MASSA, Universidad de la República (UdelaR)/ Facultad de Ciencias; ROBERTO DOCAMPO ROMERO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PABLO ANDRES RODRIGUEZ BRUNO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CARLOS DANILO CABRERA BOLOGNA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ROBERTO JOSE ZOPPOLO GOLDSCHMIDT, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Micorrización de portainjertos de manzano micropropagados. [Mycorrhization of micropropagated apple rootstocks.] |
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Fecha de publicación : |
2016 |
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Fuente / Imprenta : |
Cultivos Tropicales, 2016, v. 37, Supl. 1, p. 7-12. |
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Serie : |
1819-4087 |
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DOI : |
10.13140/RG.2.1.1451.4804 |
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Idioma : |
Español |
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Notas : |
NÚMERO ESPECIAL. Este número de la revista está dedicado al X Congreso Internacional de Biotecnología Vegetal (BioVeg2015). Article history: Recibido: 15 de mayo de 2015;
Aceptado: 3 de diciembre de 2015 |
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Contenido : |
RESUMEN
La micropropagación mediante cultivo in vitro de plantas permite la producción a gran escala de individuos genéticamente idénticos al material de partida. Las especies leñosas tienen dificultades en la etapa de aclimatación por la lentitud que presentan en el desarrollo de respuestas fisiológicas y morfológicas a los cambios de ambiente. El éxito final de la propagación in vitro depende de la capacidad de transferencia de las plantas desde el laboratorio a las condiciones de invernáculo. Una de las herramientas para contrarrestar las pérdidas durante la aclimatación, es la utilización de hongos micorrízicos arbusculares (HMA), que establecen asociaciones simbióticas mutualistas no específicas con el 90 % de las plantas vasculares. Los HMA, por su acción como agentes de biorregulación del crecimiento, biofertilizantes o biocontrol, han tenido especial atención en el manejo y propagación de las plantas frutícolas. En este trabajo se presentan los efectos de la inoculación con HMA en el inicio de la aclimatación. Para la micorrización se empleó un solo tipo de inóculo de HMA sobre dos portainjertos de manzano: un clon de M9 y otro de la serie Cornell-Geneva (RN29 y Geneva®41, respectivamente) en tres sustratos diferentes. Los plantines inoculados con micorrizas presentaron mayor expansión de sus hojas, mayor diámetro y mayor altura, respecto al control, mostrando diferencias significativas. La aclimatación se redujo de 60 a 40 días. La incorporación de este tipo de tecnología, podría generar beneficios orientados a un manejo sustentable de la producción de plantas con menor uso de agroquímicos.
.-.-.-.-.-.-.-.-.-.-.-.-.-.-.-.-.--.-.-.
ABSTRACT
Micropropagation through in vitro plant cultivation allows large-scale production of identical individuals genetically to the starting material. Woody species have difficulties in the acclimatization stage due to their slowness in the development of physiological response to environmental changes. The ultimate success of in vitro propagation depends on the capacity of plants to adapt in the moment of transfering from the laboratory to the greenhouse conditions. One of the tools to offset losses during acclimatization is the use of arbuscular mycorrhizal fungi (AMF), which sets mutualistic symbiotic associations unspecific with 90 % of vascular plants. AMF, because of their action as agents of growth bioregulation as bio-fertilizers or biocontrollers have received special attention in handling and propagation of fruit plants. In this work the effects of inoculation with AMF at the start of acclimatization are presented to mycorrhization. Inoculation with one type of AMF over two rootstocks of apple was done in a clone of M9 and one rootstock of the Cornell-Geneva series (RN29 and Geneva®41 respectively) set in three different substrates. Seedlings inoculated with AMF when compared to the control, presented further expansion of their leaves, bigger diameter and greater height, all significantly different. Acclimatization period was reduced from 60 to 40 days. The incorporation of this type of technologies could generate a more sustainable management of plant production with less use of agrochemicals. MenosRESUMEN
La micropropagación mediante cultivo in vitro de plantas permite la producción a gran escala de individuos genéticamente idénticos al material de partida. Las especies leñosas tienen dificultades en la etapa de aclimatación por la lentitud que presentan en el desarrollo de respuestas fisiológicas y morfológicas a los cambios de ambiente. El éxito final de la propagación in vitro depende de la capacidad de transferencia de las plantas desde el laboratorio a las condiciones de invernáculo. Una de las herramientas para contrarrestar las pérdidas durante la aclimatación, es la utilización de hongos micorrízicos arbusculares (HMA), que establecen asociaciones simbióticas mutualistas no específicas con el 90 % de las plantas vasculares. Los HMA, por su acción como agentes de biorregulación del crecimiento, biofertilizantes o biocontrol, han tenido especial atención en el manejo y propagación de las plantas frutícolas. En este trabajo se presentan los efectos de la inoculación con HMA en el inicio de la aclimatación. Para la micorrización se empleó un solo tipo de inóculo de HMA sobre dos portainjertos de manzano: un clon de M9 y otro de la serie Cornell-Geneva (RN29 y Geneva®41, respectivamente) en tres sustratos diferentes. Los plantines inoculados con micorrizas presentaron mayor expansión de sus hojas, mayor diámetro y mayor altura, respecto al control, mostrando diferencias significativas. La aclimatación se redujo de 60 a 40 días. La incorporación de este tipo de tec... Presentar Todo |
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Palabras claves : |
ADAPTATION; APPLE; VEGETATIVE PROPAGATION. |
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Thesagro : |
ADAPTACION; FRUTALES; MANZANO; PROPAGACION VEGETATIVA; SIMBIOSIS. |
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Asunto categoría : |
-- |
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URL : |
http://scielo.sld.cu/pdf/ctr/v37s1/ctr01s116.pdf
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Marc : |
LEADER 04329naa a2200313 a 4500
001 1056408
005 2017-01-02
008 2016 bl uuuu u00u1 u #d
024 7 $a10.13140/RG.2.1.1451.4804$2DOI
100 1 $aCASTILLO, A.
245 $aMicorrización de portainjertos de manzano micropropagados. [Mycorrhization of micropropagated apple rootstocks.]$h[electronic resource]
260 $c2016
490 $a1819-4087
500 $aNÚMERO ESPECIAL. Este número de la revista está dedicado al X Congreso Internacional de Biotecnología Vegetal (BioVeg2015). Article history: Recibido: 15 de mayo de 2015; Aceptado: 3 de diciembre de 2015
520 $aRESUMEN La micropropagación mediante cultivo in vitro de plantas permite la producción a gran escala de individuos genéticamente idénticos al material de partida. Las especies leñosas tienen dificultades en la etapa de aclimatación por la lentitud que presentan en el desarrollo de respuestas fisiológicas y morfológicas a los cambios de ambiente. El éxito final de la propagación in vitro depende de la capacidad de transferencia de las plantas desde el laboratorio a las condiciones de invernáculo. Una de las herramientas para contrarrestar las pérdidas durante la aclimatación, es la utilización de hongos micorrízicos arbusculares (HMA), que establecen asociaciones simbióticas mutualistas no específicas con el 90 % de las plantas vasculares. Los HMA, por su acción como agentes de biorregulación del crecimiento, biofertilizantes o biocontrol, han tenido especial atención en el manejo y propagación de las plantas frutícolas. En este trabajo se presentan los efectos de la inoculación con HMA en el inicio de la aclimatación. Para la micorrización se empleó un solo tipo de inóculo de HMA sobre dos portainjertos de manzano: un clon de M9 y otro de la serie Cornell-Geneva (RN29 y Geneva®41, respectivamente) en tres sustratos diferentes. Los plantines inoculados con micorrizas presentaron mayor expansión de sus hojas, mayor diámetro y mayor altura, respecto al control, mostrando diferencias significativas. La aclimatación se redujo de 60 a 40 días. La incorporación de este tipo de tecnología, podría generar beneficios orientados a un manejo sustentable de la producción de plantas con menor uso de agroquímicos. .-.-.-.-.-.-.-.-.-.-.-.-.-.-.-.-.--.-.-. ABSTRACT Micropropagation through in vitro plant cultivation allows large-scale production of identical individuals genetically to the starting material. Woody species have difficulties in the acclimatization stage due to their slowness in the development of physiological response to environmental changes. The ultimate success of in vitro propagation depends on the capacity of plants to adapt in the moment of transfering from the laboratory to the greenhouse conditions. One of the tools to offset losses during acclimatization is the use of arbuscular mycorrhizal fungi (AMF), which sets mutualistic symbiotic associations unspecific with 90 % of vascular plants. AMF, because of their action as agents of growth bioregulation as bio-fertilizers or biocontrollers have received special attention in handling and propagation of fruit plants. In this work the effects of inoculation with AMF at the start of acclimatization are presented to mycorrhization. Inoculation with one type of AMF over two rootstocks of apple was done in a clone of M9 and one rootstock of the Cornell-Geneva series (RN29 and Geneva®41 respectively) set in three different substrates. Seedlings inoculated with AMF when compared to the control, presented further expansion of their leaves, bigger diameter and greater height, all significantly different. Acclimatization period was reduced from 60 to 40 days. The incorporation of this type of technologies could generate a more sustainable management of plant production with less use of agrochemicals.
650 $aADAPTACION
650 $aFRUTALES
650 $aMANZANO
650 $aPROPAGACION VEGETATIVA
650 $aSIMBIOSIS
653 $aADAPTATION
653 $aAPPLE
653 $aVEGETATIVE PROPAGATION
700 1 $aMONTAÑEZ MASSA, A.
700 1 $aDOCAMPO, R.
700 1 $aRODRIGUEZ, P.
700 1 $aCABRERA, D.
700 1 $aZOPPOLO, R.
773 $tCultivos Tropicales, 2016$gv. 37, Supl. 1, p. 7-12.
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INIA Las Brujas (LB) |
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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
27/11/2015 |
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Actualizado : |
02/01/2017 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
B - 1 |
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Autor : |
CASTILLO, A.; CABRERA, D.; RODRIGUEZ, P.; ZOPPOLO, R.; ROBINSON, T. |
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Afiliación : |
ALICIA MARIA CASTILLO SALLE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CARLOS DANILO CABRERA BOLOGNA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PABLO ANDRES RODRIGUEZ BRUNO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ROBERTO JOSE ZOPPOLO GOLDSCHMIDT, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; T. ROBINSON, Universidad de Cornell. |
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Título : |
In vitro micropropagation of CG41 apple rootstock. |
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Fecha de publicación : |
2015 |
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Fuente / Imprenta : |
Acta Horticulturae, 2015, no. 1083, p. 569 - 576. |
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DOI : |
http://dx.doi.org/10.17660/ActaHortic.2015.1083.76 |
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Idioma : |
Inglés |
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Notas : |
Proc. VIIIth IS on In Vitro Culture and Horticultural Breeding. Eds.: J.M. Canhoto and S.I. Correia. |
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Contenido : |
ABSTRACT.
The apple production area in Uruguay is of approximately 4000 ha, with an average fruit production of 62,000 t per year. Apple is the most important crop within deciduous fruits, and has behaved as the most demanding, in crop technology, during the last years. Local climate and soil conditions in the south of the country, where the major apple producing area is located, can generate sanitary problems to apple trees. New rootstocks of CG series were introduced from Cornell University in 1996 by the Fruit Program of the National Agricultural Research Institute (INIA - Uruguay) with the purpose of evaluating new tools to solve some of these soil problems as Eriosoma lanigerum and Phytophthora sp. The rootstocks introduced were CG 16, CG 3041 and CG 5935. CG41 was selected through the results of the first trials since this rootstock showed to be well adapted, very precocious and productive, with similar vigor to M9, tolerant to Eriosoma lanigerum, and resistant to Phytophthora sp. This work was carried out to develop a protocol of in vitro propagation of CG41 rootstock, in order to generate faster plant availability to nurseries. Cytokinin (benzyladenine and thidiazuron) effect was evaluated during the multiplication phase. The multiplication rate obtained was higher than three and there was significant difference between the treatments compared. Thidiazuron was the most effective hormone in the phase of multiplication. However, thidiazuron used in successive sub-cultures generated deformations in explants and leaves of plantlets. Therefore its incorporation had to be alternated with other growth regulators. Also different elongation media were compared and the most effective included kinetin's addition. Finally, different rooting substrates were evaluated. At this level the cut in V at the base of the plant was critical, independently of its size. MenosABSTRACT.
The apple production area in Uruguay is of approximately 4000 ha, with an average fruit production of 62,000 t per year. Apple is the most important crop within deciduous fruits, and has behaved as the most demanding, in crop technology, during the last years. Local climate and soil conditions in the south of the country, where the major apple producing area is located, can generate sanitary problems to apple trees. New rootstocks of CG series were introduced from Cornell University in 1996 by the Fruit Program of the National Agricultural Research Institute (INIA - Uruguay) with the purpose of evaluating new tools to solve some of these soil problems as Eriosoma lanigerum and Phytophthora sp. The rootstocks introduced were CG 16, CG 3041 and CG 5935. CG41 was selected through the results of the first trials since this rootstock showed to be well adapted, very precocious and productive, with similar vigor to M9, tolerant to Eriosoma lanigerum, and resistant to Phytophthora sp. This work was carried out to develop a protocol of in vitro propagation of CG41 rootstock, in order to generate faster plant availability to nurseries. Cytokinin (benzyladenine and thidiazuron) effect was evaluated during the multiplication phase. The multiplication rate obtained was higher than three and there was significant difference between the treatments compared. Thidiazuron was the most effective hormone in the phase of multiplication. However, thidiazuron used in successive sub-cult... Presentar Todo |
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Palabras claves : |
BENZYLADENINE; PROPAGATION; TISSUE CULTURE. |
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Thesagro : |
MANZANA; PORTAINJERTOS; PROPAGACION VEGETATIVA; THIDIAZURON. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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Marc : |
LEADER 02746naa a2200277 a 4500
001 1054016
005 2017-01-02
008 2015 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.17660/ActaHortic.2015.1083.76$2DOI
100 1 $aCASTILLO, A.
245 $aIn vitro micropropagation of CG41 apple rootstock.$h[electronic resource]
260 $c2015
500 $aProc. VIIIth IS on In Vitro Culture and Horticultural Breeding. Eds.: J.M. Canhoto and S.I. Correia.
520 $aABSTRACT. The apple production area in Uruguay is of approximately 4000 ha, with an average fruit production of 62,000 t per year. Apple is the most important crop within deciduous fruits, and has behaved as the most demanding, in crop technology, during the last years. Local climate and soil conditions in the south of the country, where the major apple producing area is located, can generate sanitary problems to apple trees. New rootstocks of CG series were introduced from Cornell University in 1996 by the Fruit Program of the National Agricultural Research Institute (INIA - Uruguay) with the purpose of evaluating new tools to solve some of these soil problems as Eriosoma lanigerum and Phytophthora sp. The rootstocks introduced were CG 16, CG 3041 and CG 5935. CG41 was selected through the results of the first trials since this rootstock showed to be well adapted, very precocious and productive, with similar vigor to M9, tolerant to Eriosoma lanigerum, and resistant to Phytophthora sp. This work was carried out to develop a protocol of in vitro propagation of CG41 rootstock, in order to generate faster plant availability to nurseries. Cytokinin (benzyladenine and thidiazuron) effect was evaluated during the multiplication phase. The multiplication rate obtained was higher than three and there was significant difference between the treatments compared. Thidiazuron was the most effective hormone in the phase of multiplication. However, thidiazuron used in successive sub-cultures generated deformations in explants and leaves of plantlets. Therefore its incorporation had to be alternated with other growth regulators. Also different elongation media were compared and the most effective included kinetin's addition. Finally, different rooting substrates were evaluated. At this level the cut in V at the base of the plant was critical, independently of its size.
650 $aMANZANA
650 $aPORTAINJERTOS
650 $aPROPAGACION VEGETATIVA
650 $aTHIDIAZURON
653 $aBENZYLADENINE
653 $aPROPAGATION
653 $aTISSUE CULTURE
700 1 $aCABRERA, D.
700 1 $aRODRIGUEZ, P.
700 1 $aZOPPOLO, R.
700 1 $aROBINSON, T.
773 $tActa Horticulturae, 2015, no. 1083, p. 569 - 576.
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